Composite

Part:BBa_K3861000:Design

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


PlldR-sicP-sptP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS for sicP was changed.


Source

genomic sequences were derived from either E. coli for the PlldR and sicP and sptP were derived from the registry. Together the parts were synthesized.

References